Organized over period from October 2, 1863, to June 27, 1864; mustered out over period from October 14, 1864 to June 27, 1865.
- Colonel-John Murphy, formerly major, 5th Tennessee Cavalry.
Lieutenant Colonels-John Murphy, Owen Haney.
Majors-Owen Haney, James M. Dickerson.
- Thomas J. Cypert, Co. “A”. Mustered in at Nashville, October 2, 1863. Men mainly from Wayne County. Mustered out at Nashville, October 14, 1863.
- Samuel H. Martin, William A. Harrison, Co. “B”. Mustered in at Nashville, October 15, 1863. Men mainly from Wayne County. Mustered out at Nashville, October 17, 1864.
- Andrew J. Roberts, Co. “C”. Mustered in at Nashville, January 19 and April 10, 1864. Men from Wayne, Hardin and Decatur Counties. Mustered out at Gallatin, January 21, and Nashville, May 6, 1865.
- Charles W. Shipman, Co. “D”. Enrolled at Savannah. Mustered at Nashville February 1 and April 6, 1864. Men from Hardin, Wayne and Decatur Counties. Mustered out at Nashville February 1, and May 13, 1865.
- Henry D. Hamm, Robert A. Guthrie, Co. “E”. Mustered at Clifton, April 12, 1864. Men from Wayne, Decatur, Perry and Hickman Counties. Mustered out at Nashville, May 10, 1865.
- John W. Taylor, Co. “F”. Enrolled in Perry County. Mustered at Franklin January 8, and at Clifton February 20, 1864. Mustered out at Gallatin, January 19, and Nashville, March 4, 1865.
- Joel W. Chambers Co. “G”. Enrolled at Paducah, Kentucky, with men from various West Tennessee Counties. Mustered in at Waverly Landing, February 1, 1864. Mustered out at Gallatin, January 19, and Nashville, February 1, 1865.
- George E. Huckaba, Co. “H”. Enrolled at Waynesboro and Clifton, Wayne County. Mustered at Nashville, January 19, and at Clifton, March 20, 1864. Mustered out at Gallatin, May 21, and at Nashville, April 17, 1865.
- William A. Garner, Co. “I”. Enrolled at Pulaski with men from Giles and Lawrence Counties. Mustered in at Nashville, January 19 and March 8, 1864; mustered out at Gallatin, January 21, and at Nashville, March 17, 1865.
- Andrew B. Hampton, Co. “K”. Enrolled at Paducah, Kentucky, with men from various West Tennessee counties. Mustered in at Waverly Landing, March 29, and at Nashville, June 27, 1864. Mustered out at Nashville, June 27, 1865.
The report of Adjutant General J. P. Brownlow, State of Tennessee, gives February 1, 1864, as the date of John Murphy’s commission as lieutenant colonel, and states that he was promoted to colonel July 1, 1864. However, on April 20, 1864, Murphy, at Clifton, signed a letter as major, 5th Tennessee Cavalry. In March, 1864, during Major General Nathan B. Forrest’s raid into West Tennessee and Kentucky, mention was made that Major Murphy, Commanding Post at Clifton, had sent two companies of the 2nd Tennessee Mounted Infantry to join a force which was attempting to run Forrest down. It would therefore seem that the regiment was in process of organization at Clifton at that time. Brownlow’s report gives February 15, 1864, as the date of the commission of Major Owen Haney, who was the first major of the regiment.
No further references to the regiment were found in the Official Records until November 24, 1864, when Major General George H. Thomas directed Colonel C. R. Thompson, commanding post at Johnsonville, to have the two fords of the Duck River near Johnsonville picketed by the 2nd Tennessee Mounted Infantry.
On December 10, 1864, the regiment was at Nashville, with instructions to report to Gallatin, and patrol the Cumberland River from Nashville to Carthage, and report any attempt at a crossing. On December 31, the regiment, under Colonel Murphy, was reported as part of the forces assigned to duty on the Louisville and Nashville Railroad, and was still stationed at Gallatin.
On January 21, 1865, the regiment was relieved from duty at Gallatin, and ordered to proceed to Clifton, for post duty. On March 11, the regiment, still at Clifton, was ordered to report by letter to Major General Lovell H. Rousseau, Commanding District of Tennessee. On April 21, 1865, it was still at Clifton, Tennessee, and Dyer’s Compendium states it remained there until June, when it was mustered out June 27, 1865. Dyer’s Compendium also shows the regiment as having done service in East Tennessee, but nothing was found in the Official Records to substantiate this, and the muster rolls of the regiment which were printed in Adjutant General Brownlow’s report contain no information that would indicate the regiment was ever in East Tennessee. The 2nd Tennessee Infantry Regiment, which was composed of East Tennesseans, served for a time as mounted infantry in East Tennessee. It was sometimes reported as the 2nd East Tennessee Mounted Infantry Regiment, but it had no connection with this regiment.